Dr. Nicolas Bierne (HDR)
Permanent staff researcher (CNRS – CR1)
Place Eugene Bataillon, CC065
34095 Montpellier Cedex 05 – FRANCE
Station biologique
2, rue des Chantiers
34200 SETE
Phone : +33 0(4) 67 46 33 75
Fax : +33 0(4) 67 46 33 99
Evolution, Speciation, Selection, hitchhiking, local adaptation
Population genetics, Molecular Evolution, Marine Biology
I am using molecular markers together with lab experiments, field studies and theoretical developments in order to better understand evolutionary mechanisms involved in the processes of adaptation and speciation. I am mostly studying marine invertebrates because they possess unique biological characteristics that affect their genetic diversity, structure, and evolution including high fecundities, large population sizes, external fertilization with broadcast spawning, and extended larval dispersal.
My work has addressed questions about (i) heterozygosity-fitness correlations, (ii) genetics at larval stage, (iii) marine hybrid zones, (iv) mechanisms of reproductive isolation in the sea, (v) synonymous and non-synonymous substitution rates, (vi) adaptive protein evolution, and (vii) genetic hitchhiking in subdivided populations.
List of Publications
Bierne N, Welch J, Loire E, Bonhomme F & David P (2011) The coupling hypothesis: why genome scans may fail to map local adaptation genes. Molecular Ecology, in press
Bierne N (2010) The distinctive footprints of local hitchhiking in a varied environment and global hitchhiking in a subdivided population. Evolution, 64:3254-72.
Szulkin M, Bierne N & David P (2010). Heterozygosity-Fitness-Correlations: a time for reappraisal. Evolution, 64:1202-17.
Faure B, Jollivet D, Tanguy A, Bonhomme F & Bierne N (2009). Speciation in the deep sea: multi-locus analysis of divergence and gene flow between two hybridizing species of hydrothermal vent mussels. PLoS ONE, 4: e6485.
Boon E, Faure MF & Bierne N (2009) The flow of antimicrobial genes through a genetic barrier between Mytilus edulis and M. galloprovincialis. Journal of Molecular Evolution, 68:461-74.
Faure MF, David P, Bonhomme F & Bierne N (2008) Genetic hitchhiking in a subdivided population of Mytilus edulis. BMC Evolutionary Biology 8:164.
Gérard K, Bierne N, Borsa P, Chenuil A & Féral JP (2008) Pleistocene separation of mitochondrial lineages of Mytilus spp. mussels from Northern and Southern Hemispheres and strong genetic differentiation among southern populations. Mol. Phylogenet. Evol. 49:84-91.
Tanguy A, Bierne N, Saavedra C, Pina B, Bachère E, Kube M, Bazin E, Bonhomme F, Boudry P, Boulo V, Boutet I, Cancela L, Dossat C, Favrel P, Huvet A, Jarque S, Jollivet D, Klages S, Lapègue S, Leite R, Moal J, Moraga D, Reinhardt R, Samain J-F, Zouros E, Canario A (2008) Increasing genomic information in Bivalves through new EST collections in four species: development of new genetic markers for environmental studies and genome evolution. Gene 408:27-36.
Bierne N, Tanguy A, Faure M, Faure B, David E, Boutet I, Boon E, Quere N, Plouviez S, Kemppainen P, Jollivet D, Moraga D, Boudry P & David P (2007) Mark–recapture cloning: a straightforward and cost-effective cloning method for population genetics of single-copy nuclear DNA sequences in diploids. Molecular Ecology Notes 7: 562-566.
Borsa P, Daguin C & Bierne N (2007) Genomic reticulation indicates mixed ancestry in Southern-Hemisphere Mytilus spp. mussels. Biological Journal of the Linnean Society 92: 747-754.
Faure B, Bierne N, Tanguy A, Bonhomme F & Jollivet D (2007). Evidence for a slightly deleterious effect of intron polymorphisms at the EF1alpha gene in the deep-sea hydrothermal vent bivalveBathymodiolus. Gene 406: 99-107.
Sauvage C, Bierne N, Lapegue S & Boudry P (2007). Single Nucleotide polymorphisms and their relationship to codon usage bias in the Pacific oyster Crassostrea gigas. Gene 406: 22-33.
Bierne N, Bonhomme F, Boudry P, Szulkin M & David P (2006). Fitness landscapes support the dominance theory of post-zygotic isolation in the mussels Mytilus edulis and M. galloprovincialis.Proceedings of the Royal Society B-Biological Sciences 273: 1253-1260.
Bierne N & Eyre-Walker A (2006). Variation in synonymous codon use and DNA polymorphism within the Drosophila genome. Journal of Evolutionary Biology 19: 1-11.
Galtier N, Bazin E & Bierne N (2006). GC-biased segregation of noncoding polymorphisms in Drosophila. Genetics 172: 221-228.
Bierne N & Eyre-Walker A (2004). The genomic rate of adaptive amino acid substitution in Drosophila. Molecular Biology and Evolution 21: 1350-1360.
Bierne N & Eyre-Walker A (2003). The problem of counting sites in the estimation of the synonymous and nonsynonymous substitution rates: Implications for the correlation between the synonymous substitution rate and codon usage bias. Genetics 165: 1587-1597.
Bierne N, Bonhomme F & David P (2003). Habitat preference and the marine-speciation paradox. Proceedings of the Royal Society B-Biological Sciences 270: 1399-1406.
Bierne N, Borsa P, Daguin C, Jollivet D, Viard F, Bonhomme F & David P (2003). Introgression patterns in the mosaic hybrid zone between Mytilus edulis and M. galloprovincialis. Molecular Ecology 12: 447-461.
Bierne N, Daguin C, Bonhomme F, David P & Borsa P (2003). Direct selection on allozymes is not required to explain heterogeneity among marker loci across a Mytilus hybrid zone. Molecular Ecology 12: 2505-2510.
Bierne N, David P, Boudry P & Bonhomme F (2002). Assortative fertilization and selection at larval stage in the mussels Mytilus edulis and M. galloprovincialis. Evolution 56: 292-298.
Bierne N, David P, Langlade A & Bonhomme F (2002). Can habitat specialisation maintain a mosaic hybrid zone in marine bivalves? Marine Ecology-Progress Series 245: 157-170.
Bierne N, Lenormand T, Bonhomme F & David P (2002). Deleterious mutations in a hybrid zone: can mutational load decrease the barrier to gene flow? Genetical Research 80: 197-204.
Huvet A, Balabaud K, Bierne N & Boudry P (2001). Microsatellite analysis of 6-hour-old embryos reveals no preferential intraspecific fertilization between cupped oysters Crassostrea gigas andCrassostrea angulata. Marine Biotechnology 3: 448-453.
Bierne N, Tsitrone A & David P (2000). An inbreeding model of associative overdominance during a population bottleneck. Genetics 155: 1981-1990.
Bierne N, Bezuart I, Vonau V, Bonhomme F, Bedier E & Aquacop (2000). Microsatellite-associated heterosis in hatchery-propagated stocks of the shrimp Penaeus stylirostris. Aquaculture 184: 203-219.
Bierne N, Lehnert A, Bedier E, Bonhomme F & Moore SS (2000). Screening for intron-length polymorphisms in penaeid shrimps using exon-primed intron-crossing (EPIC)-PCR. Molecular Ecology9: 233-235.
Moore SS, Wilson KJ, Whan V, Bierne N, Lehnert SA, Chu KH, Pongsomboon S & Tanssanakajon A (1999) Molecular tools for mapping the Black tiger shrimp (Penaeus monodon) genome (Abstract). Plant and Animal Genome 7, 34.
Vonau V, Ohresser M, Bierne N, Delsert C, Beuzart I, Bedier E & Bonhomme F (1999). Three polymorphic microsatellites in the shrimp Penaeus stylirostris. Animal Genetics 30: 234-235.
Bierne N, Launey S, Naciri-Graven Y & Bonhomme F (1998). Early effect of inbreeding as revealed by microsatellite analyses on Ostrea edulis larvae. Genetics 148: 1893-1906.
